DNA Loading Buffer Recipe 

10X Xylene Cyanol/Bromophenol Blue DNA loading buffer recipe 

• 0.025 g of Xylene Cyanol FF
• 0.025 g of Bromophenol Blue
  0.025 g cresol red (optional)
• 1.25 ml of 10% SDS (optional)
• 4 ml 0.5 M EDTA (optional)
• 2 ml 1 M Tris-Cl pH 7.6-8.0 (optional)
• 12.5 ml of glycerol or 8 grams sucrose
• water to 20 ml
  Store sucrose buffers at 4 or -20C.