The spring peeper (Pseudacris crucifer) is a chorus frog common in the Eastern United States. Like most frogs, the male spring peeper produces vocalizations and females evaluate potential mates based on specific properties of the vocalization.  It is possible for genetic information relevant to mating to be communicated via these calls. Through comparative genetic analysis (ISSRs and microsatellite analysis) of Louisiana populations versus local Maryland populations, we have established a profile of genetic variation between populations. Phonotaxis experiments are being done to gather female call preference data to attempt a correlation of female call preferences with the established genetic component.  Correlating call preferences with individual call data could give insight into the mechanisms of speciation in Pseudacris crucifer.